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Topic : cell biology techniques
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Posted in Job Site : C-DRIVE
 
Started by : sarang limaye, Research Associate, University of Bremen   02 23 2010 09:53:00 +0000
Industry : Teaching/Education
Activity:  25 views;  last activity : 07 06 2010 20:18:09 +0000

I am facing some problems to have very puredendritic cell cultures cn some one suggest few ideas.

 
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1 Nice method to have ~97% pure untouched monocyte culture

Nice method to have ~97% pure untouched monocyte culture

idea posted by sarang limaye Research Associate, University of Bremen

Its very difficult and long process to get pure monocyte cultures. Normally we do it by adehison to plastic surface or using magnetic beds. But in most the methods we either wash the cells very add some antibodies coupled with magnetic beds. this can activate monocytes and then all the work is spoiled. will not be good idea to come up with less expensive and efficient method where we can get pure untouched monocyte culture.

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