| Topic : cell biology techniques |
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Posted in Job Site :
C-DRIVE
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Activity:
Question posted: 02 23 2010 09:34:08 +0000,
1 answers, 146 views, last activity
07 06 2010 20:18:08 +0000
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Is it really necessary to purify viral samples before adding to any cell culture system? or having monk sample is sufficient to convince people effect observed.
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Answer modified by
SANDIP B. PATHAK, Executive- Virology, Bharat Biotech Int. Ltd, Hyderabad
| 02 26 2010 14:21:42 +0000
Not Rated
Hi sarang, I will strongly recommend you to use purified virus in cell culture system because that will give you the certainty that the CPE that you are observing after addition of sample is only because of virus and not due to bacterial contamination. Also I will suggest you to use well characterized cell line and typed virus strain.
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