sarang limaye, Research Associate, University of Bremen - Profile & Expert Views of sarang limaye, Research Associate, University of Bremen

sarang limaye's Profile

sarang limaye
Research Associate, University of Bremen
	University of Bremen confidential confidential Bremen, Germany

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Professional summary

During my PhD research I have worked extensively with glycoprotein viz. Siglec to investigate their role in recognition and activation of immune system in Prof. Soerge Kelm’s working group at University of Bremen. I got very good experience in the Biochemistry, Virology and Immunology area during this period. I have worked extensively on developing stable cell line producing recombinant protein with specific glycosylation profile in both eukaryotic and prokaryotic systems. Also I have a good hand in designing and optimisation of protein production and purification techniques. I have great experience in develop and designing cell or plate based bioassays to analyse protein expression pattern and glyco-structures on proteins with the application of different techniques like FACS, ELISA, western blotting etc. Along with this I have also a good knowledge of different immunological and cell biology techniques which includes, tissue culture, blood samples handling etc.

Current Experience: Research Associate, University of Bremen
Previous: Course co-ordinator, University of Bremen, Biochemistry Department
Student Coordinator, University of Bremen, UFT, Prof Blohm's lab
Student Research Assitant, University of Bremen
Desk Top Publishing Instructor, NICE Computer Education Institute
Education:

University of Bremen, Germany
University of Bremen
University of Pune
Advance Computing & Management Center of IndSearch, Pune, India
University of Pune
Years of Experience :

5.0 Years
Skills & Interests (keywords):

ELISA, FACS, Protein Purification, Bio-assays, Protein Purification, PCR, Cell culture, Chromatograp ...
Contribution score:

(141 points)
Contacts: 7 connections, 8 following, 3 followers
Part of communities:

Healthcare & Life Sciences Professionals |

sarang limaye's Experience


Current :	Research Associate, University of Bremen
	Teaching/Education
	Germany, Bremen
	Working from 2005
	Brief summary : • Assist Bachelor and Master Students in designing and performing experiments in order to ensure smooth running of experiments and data collection; • Maintain up-to-date record of consumables and to ensure, that level of consumables meet usage demands, equipments are well-maintained so that consumables and equipments are available while running experiments; • Assist in developing standard operation procedures (SOPs) for translational research • Other related tasks as requested by the immediate supervisor
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Previous :	Course co-ordinator, University of Bremen, Biochemistry Department
	Teaching/Education
	Germany, Bremen
	Worked from 2005 to 2009
	Brief summary : Offered Advance module in Glycobiochemistry at University of Bremen during each summer semester since April 2006. Research assistant in ‘Basics in Molecular Biology and Biochemistry’ module which is offered in every winter semester at University of Bremen since November 2005.
	Yes No Are you sure wanted to delete this experience?


Previous :	Student Coordinator, University of Bremen, UFT, Prof Blohm's lab
	Teaching/Education
	Germany, Bremen
	Worked from 2004 to 2004
	Brief summary : Worked as student assistant for the ‘’Basics in Bioinformatics’’ module offered at the University of Bremen by Prof. D. Blohm UFT, Bremen, Germany
	Yes No Are you sure wanted to delete this experience?


Previous :	Student Research Assitant, University of Bremen
	Biotechnology
	Germany, Bremen
	Worked from 2003 to 2004
	Brief summary : Worked on ‘’population genetics analysis of Brazilian crabs’’ project as student research assistant in Prof. D. Blohm’s group UFT, Bremen, Germany for duration of one year. Techniques used in this work were setting up PCR and sequencing
	Yes No Are you sure wanted to delete this experience?


Previous :	Desk Top Publishing Instructor, NICE Computer Education Institute
	Publishing
	India, Pune
	Worked from 1999 to 2000
	Brief summary : DTP Instructor at ‘NICE Computer Education Institute’, Pune India. From July 1999- July 2000
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sarang limaye's Education

University of Bremen, Germany
Ph.D/Doctorate
India
From 2005 to 2011
Brief summary : PhD Thesis: Role of CD33-related Siglecs in early recognition of tumor cells and viruses.
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University of Bremen
M.Sc
Guerney
From 2002 to 2005
Brief summary : M.Sc. Thesis: Functions of CD-33 related siglecs expressed on human monocytes & macrophages In my master thesis, I have studied the availability of the siglecs for trans-interactions and then isolated and tried to identify trans-binding partners from potential counteracting cells. As source for trans-binding partners I used K562 tumour cells. Since IL-1β and IL-6 production by mononuclear phagocytes can be induced via the recognition of Sia-residues expressed by tumour cells, indicates that Sia-specific lectins like the siglecs play role for this interactions
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University of Pune
MCM
India
From 2000 to 2002
Brief summary : MCM (Master in Computer Management) Project: Production Planning System Company Name : Madan Macro Metals & Cast Pvt. Ltd Back/Front End : MS SQL Server 7.0; VB 6.0 System Features : w Facilitate smooth functioning of production schedule and material consumption; w Reporting of production schedules, material shortage, and work in progress; w Process cumulative reports for previous and current month production status; w Management tools for analysis of production
Yes No Are you sure wanted to delete this education?

Advance Computing & Management Center of IndSearch, Pune, India
Software Project Management
India
From 2001 to 2001
Brief summary :
Yes No Are you sure wanted to delete this education?

University of Pune
B.Sc
India
From 1996 to 2000
Brief summary : Bachelor’s of Microbiology
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Research Associate, University of Bremen

Working from 2005

Course co-ordinator, University of Bremen, Biochemistry Department

Worked from 2005 to 2009

Offered Advance module in Glycobiochemistry at University of Bremen during each summer semester since April 2006. Research assistant in ‘Basics in Molecular Biology and Biochemistry’ module which is offered in every winter semester at University of Bremen since November 2005.

Student Coordinator, University of Bremen, UFT, Prof Blohm's lab

Worked from 2004 to 2004

Worked as student assistant for the ‘’Basics in Bioinformatics’’ module offered at the University of Bremen by Prof. D. Blohm UFT, Bremen, Germany

Student Research Assitant, University of Bremen

Worked from 2003 to 2004

Worked on ‘’population genetics analysis of Brazilian crabs’’ project as student research assistant in Prof. D. Blohm’s group UFT, Bremen, Germany for duration of one year. Techniques used in this work were setting up PCR and sequencing

Desk Top Publishing Instructor, NICE Computer Education Institute

Worked from 1999 to 2000

DTP Instructor at ‘NICE Computer Education Institute’, Pune India. From July 1999- July 2000

University of Bremen, Germany

PhD Thesis: Role of CD33-related Siglecs in early recognition of tumor cells and viruses.

University of Bremen

University of Pune

Advance Computing & Management Center of IndSearch, Pune, India

University of Pune

Bachelor’s of Microbiology

Offered Advance module in Glycobiochemistry at University of Bremen during each summer semester since April 2006.

Research assistant in ‘Basics in Molecular Biology and Biochemistry’ module which is offered in every winter semester at University of Bremen since November 2005.